Hamideh Salehi

Hamideh SALEHI, Professeur associé

Hamideh SALEHI

HDR, PhD Biophysics

University of Montpellier

Education

May 2019 HDR, Accreditation to supervise research (Habilitation à diriger des recherches) Doctoral School of Chemical and Biological Sciences for Health ED CBS2

2011-2013 Doctor of Philosophy (Ph.D.) Biophysics, Raman spectroscopy for single cell and tissue imaging, Bioengineering and Nanoscience Laboratory, University of Montpellier, France

2010-2011 Master «Mechanical engineering», design of new shape memory polymers. Architecture, properties and process of polymers Laboratory, Arts et métiers ParisTech, France

2001-2004 Master «Physics, Photonics», Photonic crystals simulation and design, Sharif University of Technology, University of Alzahra, Tehran, Iran

1996-2001 Bsc «Physics, Lasers», Design and fabrication of CO2 laser and N2 Laser. Beheshti University, Tehran, Iran

Professional Experience

2016-2021 Biophysics Associate Professor, University of Montpellier, France

Scientific leader of the Diagnostics and Photonics team of Bioengineering and Nanoscience Laboratory
Head of the physics-chemistry platform (EDMOS) for the evaluation of medical devices for dentistry and stomatology
Examiner doctoral school of chemical and biological science for health
Grant evaluation (Fonds de Recherche du Québec -Santé, ANR)
Reporter and editor for international scientific journals
Scientific collaborator in project Comparative study of interactions between SARS-COV-2 and human and bat cells using confocal Raman microscopy, with Immunobiology of Viral Infections team, International Center for Research in Infectious Diseases, Lyon
French team leader in joint project with Institute technology, Estonian university of life science, Cobalt metal nanopartciles effect on cancer cells, Parrot project
PhD advisor for the study “Raman microscopy to follow microstructural organization of engineered cartilage” participated with the team Stem cells, cell placiticity, Regenerative Medicine and Immunotherapies, Institute of Regenerative Medicine and Biotherapies (IRMB) Montpellier

2014-2016 ATER (Teaching Research Assistant) Bioengineering and Nanoscience Laboratory University of Montpellier, France

Raman analysist in collaborative project with Société MINAPATH ( MInéralo-NAno-PATHologie), Detection of organotin in the tubal wall of an Essure implant victim by Raman Spectrometry
Biophysics team leader in joint research with Montpellier cancer research Institut (IRCM), team « Signaling of Tumor Invasion » the penetration of anti-cancer drugs in resistant MCF7.
Designed and managed the Raman experiments in collaboration with Max Mousseron Institute of Biomolecules, Faculty of Pharmaceutical and Biological Sciences. To detect the effect of new developed anticancer molecule Diazepinones targeting Metastatic Melanoma

2013-2014 Biomedical engineering postdoctoral researcher, Beckman Laser Institute and medical clinic, University of California, Irvine, USA

Stimulated Raman configuration, optical alignment for Raman setup, live cell imaging

2011-2013 Research assistant

Plan the Raman experiment for the imaging of cells treated with glycomimetic Phostins directed against glioblastomas with the Institute of Neurosciences of Montpellier and (Phost’in SAS).
Organized and performed experiments for the detection of diamond nanoparticles in cancer cells treated with a peptide in collaboration with the University of Hasselt, Belgium and the Czech Technical University, Czech Republic.
Management and realization of Raman experiments for the study of iMTA biodentin with the Faculty of Dentistry, University of Aarhus, Denmark.
Directing experiments and participating in publications Atomic force microscopy and confocal Raman microscopy to study cellular responses after oxidative damage with phenothiazine compounds with New York University, USA

Awards and Grants

2017 Canceropole young researcher prize, Nanomedicine in Cancer; from molecules to devices
2018 Young researcher prize French Dental Association (ADF)
2018 MUSE research project, University of Montpellier: Determination of the Mechanism of Action of Diazepinones targeting Melanoma
2018 platform « Evaluation of Medical Devices for Odontology and Stomatology Evaluation of Medical Devices for Odontology and Stomatology (EDMOS) : Region Occitanie (France) and the European Regional Development Fund (ERDF) funded project – Responsible for the physicochemistry platform
2019 Cancéropôle Grand Sud-Ouest, Emergence of models and tools » BCARS microscopy for screening Drug uptakes into Cancerous Cells »
2020 Estonian-French science and technology cooperation PARROT, travel expenses for two years (120 days in total)

Key Skills

Photonic crystals, design and application in telecommunication, Laser, fiber optics shape memory polymers,
Confocal Raman Microscopy Imaging, Data Analysis (K-mean clustering, PCA Principle Component Analysis, Pearson Correlation Coefficient), deep learning, R , Python, Mathlab, Witec project plus
Cell culturing, Level2 culture room, Coherent Raman Scattering CARS/SRS, single cell level stem cell and cancer imaging
Statistics
Languages: English, French, Persian

Teaching activity

Since 2014

ELC, Optical methods to assist the dentist
ELC, Laser for dental surgery
Biomaterials and biocompatibility
Laboratory materials in Odontology
Physical properties and corrosion of polymers
Material structure and crystallography in Odontology
Nano-probe, near field and evanescent wave microscopies, medical physics Master2
Principle and application of Confocal Raman microscopy in cellular and material imaging, Doctoral School Chemical and Biological Sciences for Health
General notions on biomaterials, CES-certificate of advanced studies-Biomaterials in Odontology
Implantable biomaterials, CES Biomaterials in Odontology
Behavior and characterization of biomaterials, CES Biomaterials in Odontology

2004-2009 Physics lecturer, Sirjan, Iran

Electromagnetism and mechanics. Faculty of Mechanical and Civil Engineering, Bahonar University, Sirjan, Iran.
Electromagnetics, static physics, classical mechanics, modern physics. Faculty of Physics, Payam Nour University, Sirjan, Iran.
Electricity and magnetism, fluid mechanics, wave physics and light. Faculty of Physics, Payam Nour University, Sirjan, Iran
Quantum physics and statistical mechanics, modern physics, fluid mechanics, wave physics and light. Faculty of Physics, Payam Nour University, Sirjan, Iran.

Director and advisor of 6 PhD Thesis (18 publications), 13 master students and 3 dental students

Amel Slimani, photonic approach of dental caries
Siham AlARAG, Application of Dental Stem Cells as Anticancer Drug Transporters for Chemotherapy co-direction
Elodie Terrer, Advanced microscopy and diagnosis of the carious lesion
Jean Jean N’dre Montpellier, study of engineered cartilage using Raman microscopy
Alban Dessoutter, simulation and desgin of Brillouin microscopy on biological membrane

Direction of Masters students in Biomedical physics

2012: Alexandre DEISS : cellular apoptosis using raman microscopy
2012: Derid KEITH : Prospection of dental leucomas co study
2012: Rakotobe TOVO : Prospection of dental leucomes co study
2013: Loubna DRISSENEK : Characterization of Philips toothbrushes
2013: Samir KENDRI : Confocal Raman microscopy imaging of dental stem cell differentiation
2015: Amandine LABOULAIS : Structure and composition of the enamel-dentin junction
2016: Siham AlARAG Stage M1: Imaging of dental stem cells for transporting anticancer drugs by confocal Raman microscope
2017: Siham AlARAG Stage M2: Application of stem cells as anticancer drug transporters to reduce chemotherapy side effects
2017: Jean Jean N’DRE Raman imaging of cartilage
2018: Pierre FEHLEN, skin cancer detection
2019: Firdaouss LAKHLILI, Study of the action of Phostin (against glioblastoma) by confocal Raman microscopy
2021: Gauthier MASSONE, study of motor neurons affected by amyotrophic lateral sclerosis by confocal Raman microscopy
2021: Farah BALI, Comparison between SARS-CoV-2 and measles with confocal Raman spectroscopy

French higher education qualification, national council of universities

CNU 65 Cellular Biology N° qualification 22165271172
CNU 31 Theoretical, physical and analytical chemistry N° qualification 22131271172
CNU 85-Physical Chemistry and Engineering Applied to Health N° qualification 22285271172
CNU 87-Biological, fundamental and clinical sciences N° qualification 16287271172
CNU 28-Dense Media and Materials Qualification No. 22228271172
CNU 30-Diluted media and optics N° qualification 16230271172

Publications (ORCID N° 0000-0001-7051-8110)

Salehi, H., Ramoji, A., Mougari, S. et al. Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy. Commun Chem 5, 85 (2022).
Haq Khan A , Salehi H et al. Glucose Starvation or Pyruvate Dehydrogenase Activation Induce a Broad, ERK5-Mediated, Metabolic Remodeling Leading to Fatty Acid Oxidation. Cells 2022, 11(9), 1392
Vian*, H.Salehi* , Adsorption of proteins on TiO2 particles influences their aggregation and cell penetration, Food Chemistry 130003, 2021
S,…,Salehi.H et al. Comparability of Raman Spectroscopic Configurations: A Large Scale Cross-Laboratory Study. Anal. Chem. 2020, 92, 24, 15745–15756
E, Salehi.H et al. Assessing Cobalt Metal Nanoparticles Uptake by Cancer Cells Using Live Raman Spectroscopy. Int J Nanomedicine. 2020 Sep 24;15:7051-7062
M*, Salehi.H* et al. Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds. Sci Rep. 2020, 10, 9791
Al-Obaidi, H. Salehi, PY. Collart-Dutilleul, B. Jacquot, H. Tassery, F. J. G. Cuisinier, C. Gergely, T.Cloitre, Relationship between changes in chemical composition of enamel subsurface lesions and the emitted nonlinear optical signals: an in vitro study, Caries Research, DOI: 10.1159/000505904, 2020
Tran *, H. Salehi*, M.T. Truong, M. Sandra, J. Sadoine, B. Jacquot, F. Cuisinier, C. Chaussain and T. Boukpessi Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements, Materials 12, 2102; doi:10.3390/ma12132102 , 2019
Hamideh Salehi*, Siham Al-Arag*, Elodie Middendorp, Csilla Gergely, Frederic Cuisinier, Valerie Orti Dental Pulp Stem Cells used to deliver the anticancer drug Paclitaxel Stem Cell Research & Therapy 9:103, 2018
Al-Obaidi, H. Salehi, A. Desoutter, L. Bonnet, P.Etienne, E.Terrer, B. Jacquot, B. Levallois, H. Tassery and F. J. G. Cuisinier Chemical & Nano-mechanical Study of Artificial Human Enamel Subsurface Lesions. Scientific reports, 8:4047, 2018
Desoutter, A. Slimani, R. Salih, S. Bartelemi, F. Cuisinier, H. Tassery, H. Salehi, Cross-striation in adult and deciduous enamel measured with confocal Raman microscopy, Journal of Raman spectroscopy, DOI: 10.1002 /jrs.5555, 2018
Slimani, F. Nouioua, A. Desoutter,B. Levallois,F. J. G. Cuisinier,H.Tassery,E.Terrer, H.Salehi , Confocal Raman mapping of collagen cross-link and crystallinity of human dentin–enamel junction, Journal of Biomedical Optics 22(8), 086003 , 2017
Salehi, P-Y Collart-Dutilleul , C. Gergely , F. J. G. Cuisinier, Confocal Raman microscopy to monitor extracellular matrix during Dental Pulp Stem Cells differentiation, J Biomed Opt, 1; 20(7):76013, 2015
H., CalasBennasar.I., Durand.J.C., Middendorp.E.,Valcarcel.J., Larroque.C., Nagy.K.,Turzó.K.K., Dekany.I. and Cuisinier F. Confocal Raman spectroscopy to monitor intracellular penetration of TiO2nanoparticles, Journal of Raman Spectroscopy, 45 (2014), pages 807–813
Ranjkesh B, Chevallier J, Salehi H, Cuisinier F, Isidor F, Løvschall H, Apatite precipitation on novel fast-setting calcium silicate cement containing fluoride, Acta Biomaterialia Odontologica Scandinavica 2016; 2(1):68-78
Panayotov IV, Collart-Dutilleul PY, Salehi H, Martin M, Végh A, Yachouh J, Vladimirov B, Sipos P, Szalontai B, Gergely C, Cuisinier FJ. Sprayed cells and polyelectrolyte films for biomaterial functionalization: the influence of physical PLL-PGA film treatments on dental pulp cell behavior.Macromol Biosci. 2014 Dec;14(12):1771-82.
Salehi, L. Derely, A.-G. Vegh, J.-C. Durand, C. Gergely, C. Larroque, M.-A. Faurouxand F. J. G. Cuisinier. Label-free detection of anticancer drug paclitaxel in living cells by Confocal Raman Microscopy. Appl. Phys. Let, 102 , 113701, 2013.
Salehi , E. Middendorp, C. Gergely, F. J. G. Cuisinier. Confocal Raman data analysis to comparison of apoptotic and non-apoptotic MCF-7 cells caused by anticancer drug paclitaxel. J Biomed Opt.18(5), 056010, 2013.
Salehi, Terrer E, Panayotov I, Levallois B, Jacquot B, Tassery H, Cuisinier F. Functional mapping of human sound and carious enamel and dentin with Raman Spectroscopy .J Biophotonics, 6(10):765-74, 2012
Salehi, E. Middendorp, L. Derely, B. Jacquot, F.J.G. Cuisinier. Chimiotherapeutic subtances in living cancerous cells. ADF 1er trimestre 2012, P40
Panayotov, Terrer, H. Salehi, H. Tassery, J. Yachouh, F. J G Cuisinier, B. Levallois. In vitro investigation of fluorescence of carious dentin observed with a Soprolife® camera. Clin Oral Investig, 17(3):757-63, 2013
J-C Durand, Jacquot, H. Salehi, J. Margerit, F. J G Cuisinier. Confocal Raman microscopy and SEM/EDS investigations of the interface between the zirconia core and veneering ceramic: the influence of a liner and regeneration firing. J MATER SCI-MATER M, 23(6):1343-53, 2012
J-C Durand, Jacquot, H. Salehi, M. Fages, J. Margerit, F. J G Cuisinier. Confocal Raman microscopic analysis of the zirconia/feldspathic ceramic interface. Dent Mater. Jun;28(6):661-71, 2012
Levallois, E. Terrer, Y. Panayotov, H. Salehi, H. Tassery, P. Tramini, F. Cuisinier. Molecular structural analysis of carious lesions using micro- Raman spectroscopy. EJOS; 120(5):444-51, 2012

Proceeding

Rand Al-Obaidi,; Hervé Tassery, Thierry Cloitre, Csilla Gergely, Frédéric Cuisinier, Hamideh Salehi, Confocal Raman microscopy and non linear microscopy to study human enamel incipient lesions Proceedings of SPIE 10857-11 , 2019. SPIE photonics West, San Francisco 2019 USA (Proceeding ,Oral Presentation)
Al-Arag S, Vicente M, Gergely C, Cuisinier F, Salehi H. Confocal Raman Microscope for the study of paclitaxel resistance in MCF7 cancer cells. 14th annual meeting Canceropole Journées GSO grand sud-ouest Montpellier, 2018, 193-194
Fatima N, Cueto Diaz E, Middendorp E, Desoutter A, Salehi H, Cunin F, Cuisinier F, Collart Dutilleul P.Y. Porous Silicon Resorption Kinetics during in vitro & in vivo Bone Tissue Engineering. PSST La Grande Motte, 2018
Salehi H, Al-Arag S, Middendorp E, Gergely C, Cuisinier F, Orti V. Confocal Raman Microscope for the study of anti-cancer drug delivery by dental pulp stem cells. International Conference on BioMedical Photonics. La Grande Motte, 2018
Salehi H, Al Arag S, Middendorp E, Orti V, Gergely C, Cuisinier F. Chemotherapy side effect’s reduction via targeted stem cells therapy. Raman4Clinics Annual Scientific Meeting, Serbia 2017
Salehi H, AlArag S, Middendorp E, Gergely C, Cuisinier F. Dental pulp stem cells as anticancer drug delivery system. Nanomedicine and drug delivery, Osaka Japan, 2017
Salehi H, Collart-Dutilleul P.Y, Gergely C, Cuisinier F. Dental pulp stem cells differentiation detection using confocal Raman microscopy. 4th conference on advanced applied Raman spectroscopy Berlin, Germany, 2016
Salehi H, Collart Dutilleul P.Y, Gergely C, Cuisinier F. Dental pulp stem cells differentiation detection using confocal Raman microscopy. International workshop on diagnosis of infectious diseases by detection of microbial pathogens, Berlin, Germany, 2015
Cuisinier F, Al-Obaidi R, Cloitre T, Tassery H. Salehi H, Lasers in Dentistry XXV Confocal Raman microscopy and nonlinear microscopy to study human enamel incipient lesions. 2019, DOI : 1117/12.2508306
Fatima N, Salehi H, Cuisinier F, Cunin F, Collart-Dutilleul P.Y. Porous silicon resorption and silicic acid release during bone tissue engineering. SCOPE Paris, 2019
Salehi H, Al Arag S, Middendorp E, Gergely C, Cuisinier F, Orti V. Confocal Raman Microscopy to image targeted chemotherapy. International conference on biomedical photonics, La Grande Motte, 2018
Salehi H, AlArag S, Middendorp E, Gergely C, Cuisinier F, Orti V. Utilisation des cellules souches de la pulpe dentaire pour le transport des médicaments anti-cancereux. ADF Paris, 2018
Gulka M, Varga B., Martin Fernandez M., Salehi H, Middendorp E, Cloitre T., Cuisinier F.J.G., Cígler P, Nesladek M, Gergely C, Nanodiamonds internalization in MCF7 cells monitored by cell membrane stiffness changes and their luminescent signal. International Conference on BioMedical Photonics, La Grande Motte, 2018
Gulka M, Varga B, Salehi H, Middendorp E, Cloitre T, Cuisinier F, Cígler P, Nesládek M, Gergely C. Precise nanodiamonds localization in cellular environment. E-MRS 2017, Fall Meeting, Warsaw, Poland, 2017
Salehi H, AlArag S, Middendorp E, Gergely C, Cuisinier F, Orti V. Application of dental pulp stem cells as anticancer drug transporters for chemotherapy. SFnano, Bordeaux, 2017
Salehi H, AlArag S, Cuisinier F. Confocal Raman microscopy imaging of stem cells as anticancer drug transporter. 3e journée scientifique innovations technologiques, Montpellier, 2017
Salehi H, AlArag S, Cuisinier F. Stem cells as anticancer drug transporter : Confocal Raman microscopy imaging. Canceropole young researcher Nanomedicine in Cancer, from molecules to devices, Montauban, France, 2017
Al-Arag S, Gergely C, Cuisinier F, Salehi H. Anticancer drug delivery using Dental pulp stem cells. Forum des Jeunes Chercheurs en Odontologie, Bordeaux, 2017
Fatima N, Cueto Diaz E, Desoutter A, Salehi H, Cunin F, Cuisinier F, Collart-Dutilleul P.Y. Nanotopography to increase bone regeneration: bioresorbable porous silicon scaffold for Bone Tissue Engineering. Forum des Jeunes Chercheurs en Odontologie, Bordeaux, 2017
Gulka M, Varga B, Salehi H, Middendorp E, Cloitre T, Cuisinier F, Cígler P, Nesládek M, Gergely C. Detection and localization of luminescent nanodiamonds in cellular environment using Raman imaging. MRS, Fall Meeting and exhibit, Boston, USA, 2017
Salehi H, Collart-Dutilleul P.Y, Gergely C, Cuisinier F. Confocal Raman microscopy to image cancer and stem cells. Raman4Clinics – Summer School Clinical Biophotonics, Jena, Germany, 2016
Gulka M, Martin M, Salehi H, Varga B, Cloitre T, Cuisinier F, Cígler P, Nesládek M, Gergely C. Study of diamond particles internalization by monitoring cell membrane stiffness changes and their luminescent signal. Hasselt Diamond Workshop, SBDD XXI, Belgium, 2016
Salehi H, Hassani Z, Middendorp E, Bakalara N, Cuisinier F. Confocal Raman Microscopy for Tracing Oxaphosphinanes Phostines in Glioblastom non epithelial SNB75. 12èmes journées de cancéropole JGSO 2016 – Axe 5 « Delivery and toxicity of nanodrugs » , La Grande-Motte, 2016
Gulka M, Martin M, Salehi H, Middendorp E, Cloitre T, Cuisinier F, Nesladek M, Gergely C. Tailored internalization process of peptide coated luminescent nanodiamond particles in various cell lines, SLAS, San Diego, 2016
Salehi H. Confocal Raman microscopy to detect intracellular nanoparticles and drugs penetration. Nanoscale diagnostic methods in biology and medicine, Kutna Hora, Czech Republic, 2015
Salehi H, Al-Arag S, Middendrop E, Gergely C, Cuisinier F. Stem cells as anticancer drug delivery to reduce the chemotherapy side effect SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 1006805 , doi : 10.1117/12.2251994, 2017
Salehi H, Collart-Dutilleul P.Y, Gergely C, Cuisinier F. Dental Pulp Stem Cells (DPSCs) differentiation study by Confocal Raman. Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470O, doi : 10.1117/12.2041346, 2014
Desoutter A, Salehi H, Slimani A, Marquet P, Jacquot B, Tassery H, Cuisinier F. Structure and chemical composition of the dentin-enamel junction analyzed by Confocal Raman Microscopy. Proceedings of SPIE. SPIE photonics, San Francisco, Proc. SPIE 8929, Lasers in Dentistry XX, 892907 2014; doi : 1117/12.2039826, 2014
Rywkin S, Salehi H, Cuisinier F. The use of confocal Raman microscopy to study the effects of phenothiazine derivatives in human colon cancer cells. Proceedings of SPIE, 8939-893917, SPIE photonics, San Francisco, 2014

Contacts

Mail :hamideh.salehi@umontpellier.fr
Researchgate